Mouse monoclonal 2C 8F1 Anti-Rat IgG2c gamma chain (FITC) (ab99677)

Overview

  • Product nameMouse monoclonal 2C 8F1 Anti-Rat IgG2c gamma chain (FITC)
    See all IgG2c secondary antibodies
  • Description
    Mouse monoclonal [2C 8F1] Secondary Antibody to Rat IgG2c - gamma chain (FITC)
  • Target speciesRat
  • Specificityab99677 reacts with the γ2c heavy chain (Fc) of Rat IgG2c.
  • Tested applicationsSuitable for: ELISA, ICC/IF, Flow Cytmore details
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

Applications

Our Abpromise guarantee covers the use of ab99677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.3µg for 106 cells. ab18455-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Mouse monoclonal 2C 8F1 Anti-Rat IgG2c gamma chain (FITC) images

  • BALB/c splenocytes were incubated with Rat IgG2a isotype, Rat anti-Mouse CD22 (2D6, Rat IgG1), Rat anti-Mouse CD8 (53-6.7, Rat IgG2a), Rat anti-Mouse CD45(LCA) (I3/2.3, Rat IgG2b), Rat anti-Mouse CD24 (30-F1, Rat IgG2c), and Rat anti-Mouse CD45RC (GL24, Rat IgM). After washing, the cells were stained with ab99677 at 0.3µg/106. Small lymphocytes were gated and analyzed.
  • Top: (Negative control) ab99677 being used against BALB/c spleenocytes at 1 µg/106 cells.
    Bottom: ab99677 staining BALB/c spleenocytes incubated with rat anti-mouse CD24 (IgG2c isotype) at 1 µg/106 cells.

References for Mouse monoclonal 2C 8F1 Anti-Rat IgG2c gamma chain (FITC) (ab99677)

ab99677 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"