Mouse monoclonal 4D2D9G8 Anti-Human IgG Fc (ab31925)

Overview

  • Product name
    Mouse monoclonal 4D2D9G8 Anti-Human IgG Fc
    See all IgG secondary antibodies
  • Target species
    Human
  • Specificity
    ab31925 is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Immunogen

    Chrompure Human IgG, Fc Fragment

  • Positive control
    Human serum IgG.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    4D2D9G8
  • Isotype
    IgG1
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR169873-21 are from Tissue Culture Supernatant

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
ELISA 1/10000.

Mouse monoclonal 4D2D9G8 Anti-Human IgG Fc images

  • All lanes : Mouse monoclonal 4D2D9G8 Anti-Human IgG Fc (ab31925) at 1/500 dilution

    Lane 1 : Human serum protein
    Lane 2 : Human plasma protein

    Lysates/proteins at 100 µg per lane.

    Secondary
    HRP-conjugated Goat polyclonal to mouse IgG1

    Predicted band size : 26 kDa

References for Mouse monoclonal 4D2D9G8 Anti-Human IgG Fc (ab31925)

ab31925 has not yet been referenced specifically in any publications.

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This antibody should not react with human IgA.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry and your interest in our products.

This secondary antibody is already affinity purified and it has been tested and characterized for WB, ELISA. I regret to inform you that we have not determined the MW.

Ap...

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Thank you for your recent telephone enquiry. I can confirm that the Fc constant region of an antibody remains the same for each class of antibody. Therefore, these antibodies to IgG Fc region would detect all the isotypes of IgG; IgG1, IgG2 etc....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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