Mouse monoclonal 4E3 Anti-Human IgG1 hinge heavy chain (Alkaline Phosphatase) (ab99773)

Overview

  • Product nameMouse monoclonal 4E3 Anti-Human IgG1 hinge heavy chain (Alkaline Phosphatase)
    See all IgG1 secondary antibodies
  • Description
    Mouse monoclonal [4E3] Secondary Antibody to Human IgG1 - hinge heavy chain (AP)
  • Target speciesHuman
  • Specificityab99773 reacts with the hinge region of the heavy chain of Human IgG1 as demonstrated by ELISA. May also react with IgG from other species.
  • Tested applicationsELISA, Flow Cytmore details
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 50mM Tris, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone number4E3
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab99773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/500 - 1/1000.
Flow Cyt Use at an assay dependent dilution.

References for Mouse monoclonal 4E3 Anti-Human IgG1 hinge heavy chain (Alkaline Phosphatase) (ab99773)

This product has been referenced in:
  • Whary MT  et al. Intestinal helminthiasis in Colombian children promotes a Th2 response to Helicobacter pylori: possible implications for gastric carcinogenesis. Cancer Epidemiol Biomarkers Prev 14:1464-9 (2005). Read more (PubMed: 15941957) »

See 1 Publication for this product

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ab1927 does not cross react with mouse IgGs so it could also be a option to try.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.<...

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Antibody ab50473 does not react with mouse IgGs. This might be suitable as per your requirements.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice...

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It seems product ab1927 and ab50473, could be suitable however in order to confirm it I am now waiting, species cross reactivity data from lab. I will get back to you with more updates soon.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"