The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000.
Use at an assay dependent dilution.
References for Mouse monoclonal 4E3 Anti-Human IgG1 hinge heavy chain (HRP) (ab99774)
This product has been referenced in:
Bhattacharyya T et al. IgG1 as a potential biomarker of post-chemotherapeutic relapse in visceral leishmaniasis, and adaptation to a rapid diagnostic test. PLoS Negl Trop Dis8:e3273 (2014).
Read more (PubMed: 25340782) »