Mouse monoclonal [4E3] Anti-Human IgG1 hinge heavy chain (HRP) (ab99774)

Overview

  • Product name
    Mouse monoclonal [4E3] Anti-Human IgG1 hinge heavy chain (HRP)
    See all IgG1 secondary antibodies
  • Host species
    Mouse
  • Target species
    Human
  • Specificity
    ab99774 reacts with the hinge region of the heavy chain of Human IgG1.
  • Tested applications
    Suitable for: ELISA, ICCmore details
  • Immunogen

    Other Immunogen Type corresponding to IgG1.

  • Conjugation
    HRP

Properties

Applications

Our Abpromise guarantee covers the use of ab99774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/2000.
ICC Use at an assay dependent dilution.

References

This product has been referenced in:
  • Lenders M  et al. Characterization of drug-neutralizing antibodies in patients with Fabry disease during infusion. J Allergy Clin Immunol N/A:N/A (2018). Read more (PubMed: 29421273) »
  • Campa MJ  et al. Complement Factor H Antibodies from Lung Cancer Patients Induce Complement-Dependent Lysis of Tumor Cells, Suggesting a Novel Immunotherapeutic Strategy. Cancer Immunol Res 3:1325-32 (2015). ELISA . Read more (PubMed: 26216416) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
ELISA
To determine the IgG isotype of neutralizing anti-drug antibodies, ELISAs were performed as follows. 96-well Maxisorp ELISA plates (Thermo Scientific) were coated overnight at 4°C with 50 ng agalsidase-alfa, washed 3x with PBS, and blocked for 2h at RT with 100 µl PBS/BSA 2% (per well). Subsequently, wells were washed 5x with PBS/Tween 0.1%. Purified total IgGs from patients) were used as primary antibodies and were applied in serial dilutions (1:250 1:500 1:1,000) in PBS/BSA 2% overnight at 4°C. Next day, cells were washed again 5x PBS/Tween 0.1% and incubated for 1 h at RT with IgG isotype-specific anti-human IgG-HRP (mouse α-human IgG isotype 1 [ab99774] mouse α-human IgG isotype 2 [ab99779] rabbit α-human IgG isotype 3 [ab86253] mouse α-human IgG isotype 4 [ab99823] goat α-human IgA [ab97215] mouse α-human IgE [ab99806]). Rabbit α-human IgG isotype 3 was detected with goat α-rabbit IgG-HRP [sc-2054, Santa Cruz Biotechnology,] as secondary antibody (diluted 1:5,000).
After 5 additional washes, 100 µl 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Scientific) was added to each well, incubated for 10 min, and stopped with 2M sulfuric acid. The absorption was measured at 450 nm and ratios were calculated between Enzyme replacment therapy-naïve and -treated status.
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Verified customer

Submitted Feb 27 2018

Thank you for your inquiry.

The concentration is lot specific of these antibodies and will not be written on the bottle. I am however very happy to check this for you - I am currently checking the concentrations of the lots we are having in s...

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Following on my previous message about the secondary antibodies you are interested in, the anti-human IgG isotypes are tested on a Human IgG subclass plate (IgG1, IgG2, IgG3, and IgG4) to ensure activity and minimal cross reactivity with the other IgG ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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