Mouse monoclonal [4E3] Secondary Antibody to Human IgG1 - hinge heavy chain (FITC) (ab99772)

Overview

  • Product name
    Mouse monoclonal [4E3] Secondary Antibody to Human IgG1 - hinge heavy chain (FITC)
    See all IgG1 secondary antibodies
  • Target species
    Human
  • Specificity
    ab99772 reacts with the hinge region of the heavy chain of Human IgG1 as demonstrated by ELISA. May also react with IgG from other species.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, ELISAmore details
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

Applications

Our Abpromise guarantee covers the use of ab99772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use 1µg for 106 cells.
Flow Cyt Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

References

This product has been referenced in:
  • Wang JH  et al. Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1. Oncol Lett 13:4695-4700 (2017). WB . Read more (PubMed: 28588724) »
  • Xu B  et al. Intervertebral Disc Tissue Engineering with Natural Extracellular Matrix-Derived Biphasic Composite Scaffolds. PLoS One 10:e0124774 (2015). Histology ; Mouse . Read more (PubMed: 25894203) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

ab1927 does not cross react with mouse IgGs so it could also be a option to try.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.<...

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Antibody ab50473 does not react with mouse IgGs. This might be suitable as per your requirements.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice...

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Thank you for contacting us.

It seems product ab1927 and ab50473, could be suitable however in order to confirm it I am now waiting, species cross reactivity data from lab. I will get back to you with more updates soon.

I hope this in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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