Mouse monoclonal [5A9] Anti-Human IgG Fc (HRP) (ab7499)
Key features and details
- Mouse monoclonal [5A9] Anti-Human IgG Fc (HRP)
- Conjugation: HRP
- Host species: Mouse
- Isotype: IgG2a
- Suitable for: Sandwich ELISA, ELISA
Overview
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Product name
Mouse monoclonal [5A9] Anti-Human IgG Fc (HRP)
See all IgG secondary antibodies -
Host species
Mouse -
Target species
Human -
Tested applications
Suitable for: Sandwich ELISA, ELISAmore details -
Immunogen
These clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with IgG from human serum.
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Proclin 300
Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5A9 -
Isotype
IgG2a -
General notes
Concentration varies from lot to lot and can be provided on request. -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent dilution.
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ELISA |
Use at an assay dependent dilution.
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Notes |
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Sandwich ELISA
Use at an assay dependent dilution. |
ELISA
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab7499 has been referenced in 3 publications.
- Cheng YJ et al. The characters of antibodies against PLA2R in healthy individuals and in the patient with PLA2R associated membranous nephropathy. Eur J Med Res 28:128 (2023). PubMed: 36935517
- Padula L et al. Secreted heat shock protein gp96-Ig and OX40L-Fc combination vaccine enhances SARS-CoV-2 Spike (S) protein-specific B and T cell immune responses. Vaccine X 12:100202 (2022). PubMed: 35936992
- Mboumba Bouassa RS et al. Serum and cervicovaginal IgG immune responses against a7 and a9 HPV in non-vaccinated women at risk for cervical cancer: Implication for catch-up prophylactic HPV vaccination. PLoS One 15:e0233084 (2020). PubMed: 32421735