Application notesFluorescent Dot Immunobinding Assay (F-DIBA): 1:64 determined using a 4-8 microgram dot of human IgG1.
Particle Immunofluorescence Assay (P-IFMA): 1:32 determined using a 50 µl suspension of human IgG-agarose.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Mouse monoclonal 8c/6-39 Anti-Human IgG1 Fc (FITC) (ab50473)
This product has been referenced in:
Jefferis R et al. Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of the 2nd IUIS/WHO collaborative study. Immunol Lett31:143-68 (1992).
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