Mouse monoclonal 8c/6-39 Anti-Human IgG1 Fc (FITC) (ab50473)

Overview

  • Product nameMouse monoclonal 8c/6-39 Anti-Human IgG1 Fc (FITC)
    See all IgG1 secondary antibodies
  • Target speciesHuman
  • Specificityab50473 is specific for an epitope expressed in the Fc region of the human IgG1 antibody. The antibody does not react with human IgG2, IgG3, or IgG4.
  • Immunogen

    Fc fragment of myeloma human IgG

  • Positive controlHuman IgG1.
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% inactivated BSA, 0.01M PBS, pH 8.0
  • Concentration information loading...
  • Purification notesPurified immunoglobulin.
  • Conjugation notesThe immunoglobulin fraction of mouse ascites fluid is conjugated to fluorescein isothiocyanate (FITC).
  • ClonalityMonoclonal
  • Clone number8c/6-39
  • IsotypeIgG2a
  • General notesF/P Molar Ratio: 3 to 8 This clone is also described as HP-6091.
  • Research areas

Applications

  • Application notesFluorescent Dot Immunobinding Assay (F-DIBA): 1:64 determined using a 4-8 microgram dot of human IgG1.
    Particle Immunofluorescence Assay (P-IFMA): 1:32 determined using a 50 µl suspension of human IgG-agarose.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Mouse monoclonal 8c/6-39 Anti-Human IgG1 Fc (FITC) (ab50473)

    This product has been referenced in:
    • Jefferis R  et al. Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of the 2nd IUIS/WHO collaborative study. Immunol Lett 31:143-68 (1992). Read more (PubMed: 1371266) »

    See 1 Publication for this product

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    Thank you for contacting us.

    ab1927 does not cross react with mouse IgGs so it could also be a option to try.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.<...

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    Thank you for contacting us.

    Antibody ab50473 does not react with mouse IgGs. This might be suitable as per your requirements.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice...

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    Thank you for contacting us.

    It seems product ab1927 and ab50473, could be suitable however in order to confirm it I am now waiting, species cross reactivity data from lab. I will get back to you with more updates soon.

    I hope this in...

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