Mouse monoclonal G1 7E7 Anti-Rat IgG1 heavy chain (FITC) (ab99654)

Overview

  • Product nameMouse monoclonal G1 7E7 Anti-Rat IgG1 heavy chain (FITC)
    See all IgG1 secondary antibodies
  • Description
    Mouse monoclonal [G1 7E7] Secondary Antibody to Rat IgG1 - heavy chain (FITC)
  • Target speciesRat
  • Specificityab99654 reacts with the γ1 heavy chain (Fc) of Rat IgG1.
  • Tested applicationsSuitable for: ELISA, ICC/IF, Flow Cytmore details
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

Applications

Our Abpromise guarantee covers the use of ab99654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.3µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Mouse monoclonal G1 7E7 Anti-Rat IgG1 heavy chain (FITC) images

  • BALB/c splenocytes were incubated with Rat IgG1 isotype control, Rat anti-Mouse CD22 (2D6, Rat IgG1), Rat anti-Mouse CD8 (53-6.7, Rat IgG2a), Rat anti-Mouse CD45 (LCA) (I3/2.3, Rat IgG2b), Rat anti-Mouse CD24 (30-F1, Rat IgG2c), and Rat anti-Mouse CD45RC (GL24, Rat IgM). After washing, the cells were stained with ab99654 at 0.3µg/106. Small lymphocytes were gated and analyzed.

References for Mouse monoclonal G1 7E7 Anti-Rat IgG1 heavy chain (FITC) (ab99654)

ab99654 has not yet been referenced specifically in any publications.

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