Mouse monoclonal hIgG1 Anti-Human IgG H&L preadsorbed (ab436)

Overview

  • Product nameMouse monoclonal hIgG1 Anti-Human IgG H&L preadsorbed
    See all IgG secondary antibodies
  • Target speciesHuman
  • SpecificityhIgG1 specifically recognizes human IgG. hIgG - 100% hIgG1 - 147% hIgG2 - 150% hIgG3 - 94% hIgG4 - 2.1% hIgM - 0.6% hIgA - 0.3% Goat IgG - <0.01% Rabbit IgG - <0.01% Horse IgG - <0.01% Pig IgG - <0.01% Cow IgG - <0.01% Sheep IgG - <0.01% Affinity Constant: 2x1010 L/mol human IgG.
  • Tested applicationsSuitable for: ELISA, RIAmore details
  • Minimal
    cross-reactivity

    Cow, Goat, Horse, Pig, Rabbit, Sheep more details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.095% Sodium Azide
    Constituents: 0.9% Sodium chloride, 37mM Sodium citrate, 125mM PBS, pH 6.0
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberhIgG1
  • IsotypeIgG2a
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
RIA
  • Application notesELISA: Use at an assay dependant dilution.
    RIA: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Mouse monoclonal hIgG1 Anti-Human IgG H&L preadsorbed (ab436)

    ab436 has not yet been referenced specifically in any publications.

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    Thank you for contacting Abcam.

    We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, pleas...

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    It is unknown as to which constant region of the heavy chain this antibody recognises, so we do not know if this antibody could distinguish between the two.

    We have not tested the cross reactivity of this antibody with chimpanzee or with any other monkey IgA or with other monkey Ig classes. Its cross reativity with human IgG is below 0.3% and with human IgM(plasma) 2.6%. Human IgM(Waldenström's macroglobul...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"