Mouse monoclonal hIgG1 Anti-Human IgG H&L preadsorbed (ab436)

Overview

  • Product name
    Mouse monoclonal hIgG1 Anti-Human IgG H&L preadsorbed
    See all IgG secondary antibodies
  • Target species
    Human
  • Specificity
    hIgG1 specifically recognizes human IgG. hIgG - 100% hIgG1 - 147% hIgG2 - 150% hIgG3 - 94% hIgG4 - 2.1% hIgM - 0.6% hIgA - 0.3% Goat IgG - <0.01% Rabbit IgG - <0.01% Horse IgG - <0.01% Pig IgG - <0.01% Cow IgG - <0.01% Sheep IgG - <0.01% Affinity Constant: 2x1010 L/mol human IgG.
  • Tested applications
    Suitable for: ELISA, RIAmore details
  • Minimal
    cross-reactivity

    Cow, Goat, Horse, Pig, Rabbit, Sheep more details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.095% Sodium Azide
    Constituents: 0.9% Sodium chloride, 37mM Sodium citrate, 125mM PBS, pH 6.0
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    hIgG1
  • Isotype
    IgG2a
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
RIA
  • Application notes
    ELISA: Use at an assay dependant dilution.
    RIA: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Mouse monoclonal hIgG1 Anti-Human IgG H&L preadsorbed (ab436)

    ab436 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam.

    We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, pleas...

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    It is unknown as to which constant region of the heavy chain this antibody recognises, so we do not know if this antibody could distinguish between the two.

    We have not tested the cross reactivity of this antibody with chimpanzee or with any other monkey IgA or with other monkey Ig classes. Its cross reativity with human IgG is below 0.3% and with human IgM(plasma) 2.6%. Human IgM(Waldenström's macroglobul...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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