Mouse monoclonal HP-6017 Anti-Human IgG Fc (FITC) (ab49867)

Overview

  • Product nameMouse monoclonal HP-6017 Anti-Human IgG Fc (FITC)
    See all IgG secondary antibodies
  • Target speciesHuman
  • SpecificityThis antibody reacts specifically with IgG (Fc fragment), all subclasses. The antibody does not react with IgA, IgM, purified light chains or the Fab fragment of IgG. This clone has been evaluated for specificity using a wide range of immunological techniques in the IUIS/WHO collaborative study and has been adopted by IUIS/WHO study as a standard reagent.
  • Tested applicationsSuitable for: Dot blotmore details
  • Immunogen

    Human IgG coupled to polyaminostyrene beads.

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% BSA (inactivated), 0.01M PBS, pH 8.0
  • Concentration information loading...
  • PurityIgG fraction
  • Conjugation notesThe immunoglobulin fraction of the ascites fluid was conjugated to fluorescein isothiocyanate (FITC) and then further purified to remove unconjugated FITC. F/P Molar Ratio: 3 to 5.
  • ClonalityMonoclonal
  • Clone numberHP-6017
  • IsotypeIgG2a
  • General notes

    Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding. A certain population of the lymphocytes posseses the "Fc receptor", therefore specific immunoreagents targeted only for the Fc fragment should prove useful.

    F-DIBA (Fluorescent Dot Immunobinding Assay): Minimum dilution of 1/16 for the conjugate on a 4-8µg dot of Human IgG (Fc fragment). F-IFMA (Particle Immunofluorescent Assay): Minimum dilution of 1/16 for the conjugate on 50µl suspension of Human IgG Agarose coated with approximately 20mg Human IgG.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/16.

References for Mouse monoclonal HP-6017 Anti-Human IgG Fc (FITC) (ab49867)

ab49867 has not yet been referenced specifically in any publications.

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