Mouse monoclonal HP6025 Anti-Human IgG4 Fc (Alkaline Phosphatase) (ab99822)

Overview

  • Product nameMouse monoclonal HP6025 Anti-Human IgG4 Fc (Alkaline Phosphatase)
    See all IgG4 secondary antibodies
  • Description
    Mouse monoclonal [HP6025] Secondary Antibody to Human IgG4 - Fc (AP)
  • Target speciesHuman
  • Specificityab99822 reacts with the Fc portion of the heavy chain of Human IgG4 as demonstrated by ELISA.
  • Tested applicationsSuitable for: ELISA, WB, Dot blot, IHC-Fr, ICCmore details
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 50mM Tris, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified from Ascites fluid.
  • ClonalityMonoclonal
  • Clone numberHP6025
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/500 - 1/1000.
WB Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

References for Mouse monoclonal HP6025 Anti-Human IgG4 Fc (Alkaline Phosphatase) (ab99822)

ab99822 has not yet been referenced specifically in any publications.

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Thank you for contacting us. Each of the clones we discussed has been tested by ELISA for cross-reactivity with the other subclasses and was found to be negative. Consequently, the extra purification step of cross-adsorption was not necessary. If you h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"