Mouse monoclonal Mp 4H2 Anti-Human IgG heavy chain (ab9243)

Overview

  • Product nameMouse monoclonal Mp 4H2 Anti-Human IgG heavy chain
    See all IgG secondary antibodies
  • Target speciesHuman
  • SpecificityThis antibody is specific for the gamma heavy chain of human IgG
  • Tested applicationsSuitable for: ELISA, IHC-Fr, WBmore details
  • Immunogen

    Purified intact human IgG

  • Positive controlnormal human serum Ig Fraction

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituent: PBS
  • Concentration information loading...
  • PurityAscites
  • ClonalityMonoclonal
  • Clone numberMp 4H2
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent dilution.
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 25, 50 -150 kDa. The antibody recognizes the heavy chains of human IgG. The size of the band observed depends on the sample preparation and degree of fragmentation the Ig molecule has undergone. Under reduced conditions in WB heavy chains at 50 kD. Intact (native) IgG is 150 kDa. Therefore either 50 kDa or the entire IgG at 150kDa will be observed, depending on how much the molecule is fragmented (you may see both).

References for Mouse monoclonal Mp 4H2 Anti-Human IgG heavy chain (ab9243)

This product has been referenced in:
  • Gieffers C  et al. APG350 induces superior clustering of TRAIL receptors and shows therapeutic antitumor efficacy independent of cross-linking via Fc? receptors. Mol Cancer Ther 12:2735-47 (2013). Read more (PubMed: 24101228) »

See 1 Publication for this product

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We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, pleas...

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Ab9243 has been used to identify human IgG in ELISA. The antibody's originator has not seen any cross-reaction to other isotypes. If you have any more questions please contact us again.

This antibody is specific for the human IgG heavy chain, but its epitope has not been mapped further. However, this antibody has been used to test chimeric antibodies where the Fab region is human. As ab9243 works well for this testing, one can assume ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"