Mouse monoclonal [SB108a] Anti-Rhesus monkey IgG heavy chain (HRP) preadsorbed (ab99707)

Overview

  • Product name
    Mouse monoclonal [SB108a] Anti-Rhesus monkey IgG heavy chain (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Mouse
  • Target species
    Rhesus monkey
  • Specificity
    ab99707 reacts with Rhesus and Cynomolgous Monkey IgG heavy chain as demonstrated by ELISA.
  • Tested applications
    Suitable for: ELISAmore details
  • Minimal
    cross-reactivity

    Cat, Chicken, Cow, Donkey, Goat, Guinea pig, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • Immunogen

    Rhesus IgG

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: 50% PBS, 50% Glycerol, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    SB108a
  • Isotype
    IgG1
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/2000. Also suitable for fluorescent ELISA.

References

ab99707 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ELISA
We wanted to test the mouse monoclonal SB108a Anti-Rhesus monkey IgG heavy chain (HRP) preadsorbed (ab99707) to test its reactivity with IgG from Aotus nancymaae, a new world primate. The plan is to perform ELISA using this as the secondary antibody to detect anti-human IgG in the Aotus serum. Because the capture reagent is human IgG, we need this antibody to be minimally reactive with human IgG, while being reactive to the Aotus anti-human IgG. Aotus and rhesus IgGs share high levels of sequence homology.
We performed a direct ELISA with the mouse monoclonal SB108a Anti-Rhesus monkey IgG heavy chain (HRP) preadsorbed (ab99707) coating the plate with either Rhesus macaquesera, Aotus nancymaae sera and human sera. As expected, there was a detectable signal by ELISA for the rheusus sera-coated wells and not for the human sera-coated wells. Unfortunately for our intended purposes, there was no cross-reactivity with the Aotus nancymaae sera.
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Verified customer

Submitted Jan 31 2018

To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species: Cat, Chicken, Cow, Donkey, Goat, Guinea pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Pre-adsorption (also referred to as cros...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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