Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)
Key features and details
- Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Mouse
- Isotype: IgG2b
- Suitable for: ELISA, WB
Related conjugates and formulations
Overview
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Product name
Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)
See all IgG Light chain secondary antibodies -
Host species
Mouse -
Target species
Rabbit -
Specificity
ab99696 reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50 kD). -
Tested applications
Suitable for: ELISA, WBmore details -
Immunogen
Full length protein corresponding to Rabbit IgG Light chain. Rabbit Light Chain
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.0095% Magnesium chloride, 0.605% Tris -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SB62a -
Isotype
IgG2b -
Light chain type
lambda -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/2000 - 1/4000.
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WB |
Use at an assay dependent concentration.
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Notes |
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ELISA
1/2000 - 1/4000. |
WB
Use at an assay dependent concentration. |
Images
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Western blot showing that Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) specifically binds to light-chains
Left blot: Coomassie stain showing the rabbit heavy (~50kDa) and light-chains (~25 kDa)
Lane 1: 10 µg rabbit monoclonal was loaded
Lane 2: 5 µg rabbit monoclonal was loaded
Lane 3: 2 µg rabbit monoclonal was loadedRight blot: WB using Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) as secondary antibody at 1/10,000
Lane 1: 1 µg rabbit monoclonal was loaded
Lane 2: 0.5 µg rabbit monoclonal was loaded
Lane 3: 0.25 µg rabbit monoclonal was loaded -
All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/5000 dilution
Lanes 3 - 4 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/20000 dilution
Lanes 5 - 6 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab99696 has been referenced in 2 publications.
- Ao X et al. lincRNA-p21 inhibits the progression of non-small cell lung cancer via targeting miR-17-5p. Oncol Rep 41:789-800 (2019). PubMed: 30535441
- Castro-Severyn J et al. Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study. Front Microbiol 10:2161 (2019). PubMed: 31611848