Mouse monoclonal SB62a Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)

Overview

  • Product nameMouse monoclonal SB62a Anti-Rabbit IgG light chain (Alkaline Phosphatase)
    See all IgG Light chain secondary antibodies
  • Target speciesRabbit
  • Specificityab99696 reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50 kD).
  • Tested applicationsELISA, WBmore details
  • Immunogen

    Rabbit Light Chain

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 50mM Tris, 1mM Magnesium chloride, pH 8
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Monoclonal
  • Clone numberSB62a
  • IsotypeIgG2b
  • Light chain typelambda
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab99696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/4000.
WB Use at an assay dependent concentration.

Mouse monoclonal SB62a Anti-Rabbit IgG light chain (Alkaline Phosphatase) images

  • Western blot showing that Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) specifically binds to light-chains


    Left blot: Coomassie stain showing the rabbit heavy (~50kDa) and light-chains (~25 kDa)

    Lane 1: 10 µg rabbit monoclonal was loaded
    Lane 2: 5 µg rabbit monoclonal was loaded
    Lane 3: 2 µg rabbit monoclonal was loaded

    Right blot: WB using Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) as secondary antibody at 1/10,000

    Lane 1: 1 µg rabbit monoclonal was loaded
    Lane 2: 0.5 µg rabbit monoclonal was loaded
    Lane 3: 0.25 µg rabbit monoclonal was loaded

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/5000 dilution
    Lanes 3 - 4 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/20000 dilution
    Lanes 5 - 6 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.
    Predicted band size : 42 kDa
    Observed band size : 42 kDa

    Exposure time : 12 minutes


References for Mouse monoclonal SB62a Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)

ab99696 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"