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|Sample type||Average %||Range|
|Cell culture supernatant||105.5||96% - 112%|
|Plasma||86.89||68% - 105%|
Abcam’s MPO (Myeloperoxidase) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse MPO (Myeloperoxidase) in plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MPO coated on a 96-well plate. Standards and samples are pipetted into the wells and MPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.
We have not been able to detect the endogenous Mouse MPO (Myeloperoxidase) in normal serum with ab155458, only in serum spiked with Mouse MPO (Myeloperoxidase).
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-mouse MPO||2 vials|
|MPO Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse MPO Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab155458 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|