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|Sample type||Average %||Range|
|Plasma||100.8||89.1% - 112%|
Abcam’s Total PAI1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of active plasminogen activator inhibitor type 1 in mouse plasma.
Mouse PAI1 present in plasma reacts with the capture antibody coated and dried on a microtiter plate. Free, latent and complexed PAI1 will bind to the plate. Any unbound PAI1 is washed away and an anti-PAI1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the total PAI1 present in the samples, is then reacted with the secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of total PAI1 in the sample.
|Components||1 x 96 tests|
|10X Wash Buffer||1 x 50ml|
|Anti-mouse PAI1 coated Microtiter Plate||1 unit|
|Anti-mouse PAI1 Primary Antibody||1 vial|
|Anti-rabbit horseradish peroxidase secondary antibody||1 x 10µl|
|Mouse PAI1 Standard||1 vial|
|TMB substrate solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab157529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab157529 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"