|Sample type||Average %||Range|
|Cell culture supernatant||76.85||68% - 87%|
|Serum||82.47||73% - 94%|
|Plasma||76.78||67% - 85%|
Abcam’s Prolactin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Prolactin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Prolactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Prolactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-mouse Prolactin (lyophilized)||2 vials|
|Prolactin Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse Prolactin Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|