• Product name
    Mouse Prolactin ELISA Kit
    See all Prolactin kits
  • Detection method
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 30 pg/ml
  • Range
    27.43 pg/ml - 20000 pg/ml
  • Recovery

    81 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 76.85 68% - 87%
    Serum 82.47 73% - 94%
    Plasma 76.78 67% - 85%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Prolactin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Prolactin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Prolactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Prolactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-mouse Prolactin (lyophilized) 2 vials
    Prolactin Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse Prolactin Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation.
  • Cellular localization
  • Alternative names
    • AV290867
    • Lactogenic Hormone
    • Luteotropic Hormone
    • Mammotropin
    • OTTMUSP00000000651
    • PRL
    • Prl1a1
    • Prol
    • Prolactin precursor
    • RNPROL
    • RP23-351I6.2
    • RP3-404K8.1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Prolactin measured in mouse biological fluids showing quantity (pg) per mL of tested sample. Samples diluted 10-270 fold.

  • Representative standard curve using ab100736

  • Representative standard curve using ab100736



This product has been referenced in:
  • Golub Y  et al. Effects of In utero environment and maternal behavior on neuroendocrine and behavioral alterations in a mouse model of prenatal trauma. Dev Neurobiol 76:1254-1265 (2016). Read more (PubMed: 26899175) »
  • O'Hara L  et al. Pituitary androgen receptor signalling regulates prolactin but not gonadotrophins in the male mouse. PLoS One 10:e0121657 (2015). Mouse . Read more (PubMed: 25799562) »

See all 4 Publications for this product

Customer reviews and Q&As

To determine the prolactin (PRL) contents of the pituitary gland, we extracted PRL from the pituitary gland of mice on 19th day of gestation by using 1 M urea/PBS solution. PRL levels were measured by using a ELISA kit (ab100736, lot no. GR291799-1 Abcam).
We detected the pituitary PRL contents with this ELISA kit.
Moreover, when the anterior pituitaries were homogenized and frozen-thawed in PBS (pH 7) containing 1 M urea, yields of PRL was maximum rather than in PBS only. This procedure would be the best for the estimation of PRL.

Sir or Madam シモカワ

Verified customer

Submitted Feb 08 2018

Wir empfehlen eine 5- 100 fache Verdünnung für Serumproben. Da der Level an Zielprotein sehr variieren kann zwischen verschiedenen Proben muss der optimale Verdünnungsfaktor experimentell vom Benutzer herausgefunden werden. Wir...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for contacting us. I apologize for any inconvenience.

I can confirm that the spiky bottomed vial is Item-F (Biotinylated anti mouse antibody) and the round bottomed vials Item C are recombinant prolacticn.

Please let me kno...

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I am sorry you received a kit which contained a contaminated buffer and for any trouble this has caused. As requested, I have issued a free of charge replacement for one kit of ab100736.

To check the status of the order please contact our Cu...

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Thanks so much for all your troubleshooting efforts and for sending along the pictures. I agree that whatever that is does not look like precipitation. I would be happy to send you a replacement of ab100736.

If you could, please send me the P...

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Unless there is some discoloration I don’t think you have much to worry about especially if the standard curve looks fine as it is likely just precipitation. Do you have a photo you could send me?

As the diluent does contain proteins a...

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Thank you for your inquiry. Please see my answers to your questions below: 1. I can confirm that there are special Serum collection tubes commercially available from many vendors. But serum can also easily be extracted by your customer by hand....

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