Mouse proMMP9 ELISA Kit (ab100732)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 74.07 pg/ml - 18000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse proMMP9 ELISA Kit
See all MMP9 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 50 pg/ml -
Range
74.07 pg/ml - 18000 pg/ml -
Recovery
100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 109.7 96% - 118% Serum 114.4 97% - 132% Plasma 112 101% - 121% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
proMMP9 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100732) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse proMMP9 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse proMMP9 coated on a 96-well plate. Standards and samples are pipetted into the wells and proMMP9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse proMMP9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of proMMP9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Mouse proMMP9 2 vials proMMP9 Microplate (12 x 8 wells) 1 unit Recombinant Mouse proMMP9 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
Tissue specificity
Produced by normal alveolar macrophages and granulocytes. -
Involvement in disease
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Alternative names
- 82 kDa matrix metalloproteinase-9
- 92 kDa gelatinase
- 92 kDa type IV collagenase
see all -
Database links
- Entrez Gene: 17395 Mouse
- SwissProt: P41245 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab100732 has been referenced in 5 publications.
- Boodhoo K et al. Dysregulated healing responses in diabetic wounds occur in the early stages postinjury. J Mol Endocrinol 66:141-155 (2021). PubMed: 33350981
- Deng HJ et al. A20 Establishes Negative Feedback With TRAF6/NF-κB and Attenuates Early Brain Injury After Experimental Subarachnoid Hemorrhage. Front Immunol 12:623256 (2021). PubMed: 34381441
- Ma J et al. Rivaroxaban attenuates thrombosis by targeting the NF-?B signaling pathway in a rat model of deep venous thrombus. Int J Mol Med 40:1869-1880 (2017). PubMed: 29039441
- Yan B et al. HMGB1-TLR4-IL23-IL17A axis promotes paraquat-induced acute lung injury by mediating neutrophil infiltration in mice. Sci Rep 7:597 (2017). PubMed: 28377603
- Xie J et al. The effects of interleukin-1ß in modulating osteoclast-conditioned medium's influence on gelatinases in chondrocytes through mitogen-activated protein kinases. Int J Oral Sci 7:220-31 (2015). PubMed: 26674424