Mouse Prostasin ELISA Kit (ab213884)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 0.78 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Prostasin ELISA Kit
See all PRSS8 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 1.504ng/ml 0.102 = 6.8% 2 16 6.502ng/ml 0.436 = 6.7% 3 16 25.206ng/ml 1.205 = 4.6% Inter-assay Sample n Mean SD CV% 1 24 0.113ng/ml 0.113 = 6.9% 2 24 0.484ng/ml 0.484 = 7.2% 3 24 1.723ng/ml 1.723 = 6.3% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 50 pg/ml -
Range
0.78 ng/ml - 50 ng/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse Prostasin Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213884) is designed for the quantitative measurement of Mouse Prostasin in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for Prostasin has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A30-Q289) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Prostasin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Prostasin amount of sample captured in plate.
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Notes
Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse Prostasin coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Mouse Prostasin antibody 1 x 100µl Lyophilized recombinant Mouse Prostasin standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Possesses a trypsin-like cleavage specificity. -
Tissue specificity
Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Cellular localization
Cell membrane and Secreted > extracellular space. Found in the seminal fluid. Secreted after cleavage of its C-terminus. - Information by UniProt
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Alternative names
- 2410039E18Rik
- AI313909
- C79772
see all -
Database links
- Entrez Gene: 76560 Mouse
- SwissProt: Q9ESD1 Mouse
- Unigene: 5875 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213884 has not yet been referenced specifically in any publications.