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|Sample type||Average %||Range|
|Cell culture supernatant||106.5||95% - 115%|
|Serum||95.05||83% - 104%|
|Plasma||89.2||74% - 104%|
Abcam’s RANKL Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse RANKL in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse RANKL coated on a 96- well plate. Standards and samples are pipetted into the wells and RANKL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse RANKL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANKL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Mouse RANKL (lyophilized)||2 vials|
|RANKL Microplate (12 strips x 8 wells)||1 unit|
|Recombinant Mouse RANKL Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100749 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"