Overview

Description

  • NatureSynthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab38371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-REC8 antibody (ab38372)

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Cohesin Rec8p
    • Human homolog of rad21 S. pombe
    • Meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family
    • Meiotic recombination protein REC8 homolog
    • Meiotic recombination protein REC8-like 1
    • rec8
    • REC8 homolog (yeast)
    • REC8 like 1
    • REC8_HUMAN
    • Rec8p
    • Recombination and sister chromatid cohesion protein homolog
    see all
  • FunctionRequired during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
  • Tissue specificityExpressed in testis and thymus.
  • Sequence similaritiesBelongs to the rad21 family.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationNucleus. Chromosome. In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II.
  • Information by UniProt

References for Mouse REC8 peptide (ab38371)

ab38371 has not yet been referenced specifically in any publications.

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