Overview

  • Product name
    Mouse Resistin ELISA Kit
    See all Resistin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 8 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 7.5%
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    6.5 pg/ml
  • Range
    31.25 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 91 88% - 95%
    Cell culture media 100 98% - 103%
    Heparin Plasma 104 100% - 111%
    EDTA Plasma 95 90% - 103%
    Citrate Plasma 109 106% - 115%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Rabbit, Guinea pig, Hamster, Dog, Human, Pig
  • Product overview

    Abcam’s Resistin (RETN) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Resistin protein in mouse serum, plasma, cell culture supernatants, cell and tissue extracts.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

    Sensitivity:

    Samples diluted in Sample Diluent NS: 6.5 pg/mL.

    Samples diluted in 1X Cell Extraction Buffer PTR: 27.4 pg/mL.

  • Notes

    Mouse Resistin (RETN), also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone), is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family. Mouse Resistin is a 114-amino acid (aa) peptide (with a 20 aa signal sequence and a 94 aa mature segment) containing 11 cysteines that allow the association of several Resistin monomers into macromolecular complexes. Mouse Resistin is 75% and 56% identical to rat and human Resistin, respectively.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab205574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve comparison between mouse Resistin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Native mouse Resistin was measured in 2.5% mouse serum diluted in a 2-fold dilution series in Sample Diluent NS. Recombinant mouse Resistin was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • Native mouse Resistin was measured in 2.5% mouse plasma citrate, mouse plasma EDTA, and mouse plasma heparin samples diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • Native mouse Resistin was measured in 10 μg/mL of 3T3-L1 adipocyte cell extract diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • 3T3-L1 cells were cultured in the presence and absence 1 µM Dexamethasone, 0.5 mM IBMX, 1 µg/mL insulin for 10 days to generate adipocytes and undifferentiated cells respectively. At the end of the treatment, protein extraction was carried out according to section 11.4 of the booklet. Both 3T3-L1 adipocytes and undifferentiated cell extracts were measured in duplicate at 10 µg/mL using this kit. The Raw O.D. values for each sample are graphed, with the background O.D. shown as the dashed line. The 3T3-L1 adipocytes were measured at 179 pg of Resistin/µg of cell lysate, whereas the 3T3-L1 undifferentiated cells measured below the limit of detection for this assay.

Protocols

References

ab205574 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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