Overview

  • Product name
    Anti-Mouse Serum Albumin antibody
    See all Mouse Serum Albumin primary antibodies
  • Description
    Goat polyclonal to Mouse Serum Albumin
  • Tested applications
    Suitable for: ICC, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control
    • This antibody gave a positive signal in human and mouse liver tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab19194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/1000 - 1/10000.
ELISA 1/100 - 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IHC-P Use at an assay dependent dilution.

Target

  • Function
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity
    Plasma.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alb 1 antibody
    • alb antibody
    • ALBU_MOUSE antibody
    • Albumin 1 antibody
    • Albumin antibody
    • Serum albumin antibody
    see all

Images

  • All lanes : Anti-Mouse Serum Albumin antibody (ab19194) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 69 kDa


    Exposure time : 1 minute
  • ab19194 staining Mouse Serum Albumin (red) in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 1% normal donkey serum, 0.1% bovine serum albumin, 0.1% NaN3, 0.3% Triton X-100 in PBS for 1 hour at room temperature; antigen retrieval was using a citrate buffer. Samples were incubated with primary antibody and an AlexaFluor®555-conjugated IgG was used as the secondary antibody.

References

This product has been referenced in:
  • Park JH  et al. Transient cerebral ischemia induces albumin expression in microglia only in the CA1 region of the gerbil hippocampus. Mol Med Rep 16:661-665 (2017). IHC . Read more (PubMed: 28586018) »
  • Kim Y  et al. Three-dimensional (3D) printing of mouse primary hepatocytes to generate 3D hepatic structure. Ann Surg Treat Res 92:67-72 (2017). Read more (PubMed: 28203553) »

See all 23 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.
As we haven't tested the cross-reactivity for this antibodies, it is very much likely that they will react with other albumins.
Therefore, I would suggest to use milk as a blocking agent (0.5% Skim milk/PBS for 1 h...

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Thank you for your response.

It would be much appreciated if I could get some more information which would help me identify the source of the problem. Could you provide some further details of the protocol used and complete the following form...

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Thank you for your enquiry regarding ab19194 and your interest in our products.

I can confirm that we have used BSA as our blocking agent for our in-house testings.

I hope this helps and if I can assist further, please do not hesita...

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Thank you for contacting us. Serum albumin may be a suitable loading control, but I am not sure if levels will vary from one animal to another, for example healthy vs. diseased. These three antibodies detect mouse serum albumin: ab19194...

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Thank you for your inquiry. I would not recommend storing this antibody frozen. We have not tested this antibody after it's been frozen so we can't say how it would affect the stability of the antibody. Plus, this antibody does not have any cryopr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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