Mouse skeletal muscle tissue lysate - total protein (ab29711)

Overview

  • Product nameMouse skeletal muscle tissue lysate - total protein
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Sodium Deoxycholate, Tergitol-NP40, EDTA, Magnesium chloride, HEPES, Sucrose, Potassium chloride, Protease inhibitor, 0.15M Beta mercaptoethanol, pH 7.9
  • Concentration information loading...
  • Lysate notesProtein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab29711 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    References for Mouse skeletal muscle tissue lysate - total protein (ab29711)

    ab29711 has not yet been referenced specifically in any publications.

    Product Wall


    I have heard back from the lab concerning donor information for the lots requested of human, mouse and rat skeletal tissue lysates.


    Skeletal Muscle (Human) Tissue Lysate: 24 year old normal male

    Skeletal Muscle (Rat) Tiss...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"