Mouse skin tissue lysate (7 days) (ab7273)

Overview

  • Product nameMouse skin tissue lysate (7 days)
    See all Skin lysates
  • Description
    Mouse skin lysate
  • General notesSource: Outbred NIH-Swiss Albino mice (7 days old)


    The mouse skin tissue was collected from seven (7) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b mercaptoethanol.

  • Tested applicationsSuitable for: WBmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab7273 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent concentration. Mouse skin tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

    References for Mouse skin tissue lysate (7 days) (ab7273)

    ab7273 has not yet been referenced specifically in any publications.

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