The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
|50x DAB Chromogen||1 x 0.5ml|
|Biotinylated goat anti-mouse IgG(H+L)||1 x 15ml|
|DAB substrate||1 x 15ml|
|Hydrogen Peroxide Block||1 x 15ml|
|Protein block||1 x 15ml|
|Streptavidin Peroxidase||1 x 15ml|
Our Abpromise guarantee covers the use of ab64259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
Immunohistochemical analysis staining SMA or CD68 in paraffin embedded mouse liver tissue sections Samples were incubated with anti-α- SMA (ab7817) or anti-CD68 (ab49777). A Mouse specific HRP/DAB (ABC) Detection IHC Kit (AB64259 was used as a stain and Mayer’s hematoxylin was used as a counterstain. Top panel of images staining CD68 and bottom panel staining α-SMA.