Mouse specific HRP/DAB (ABC) Detection IHC Kit (ab64259)

Overview

  • Product nameMouse specific HRP/DAB (ABC) Detection IHC Kit
    See all HRP/DAB detection kit kits
  • Product overview

    The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

  • Tested applicationsSuitable for: IHC-Pmore details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 15 ml
    50x DAB Chromogen 1 x 0.5ml
    Biotinylated goat anti-mouse IgG(H+L) 1 x 15ml
    DAB substrate 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml
  • Research areas
  • RelevanceHRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Applications

Our Abpromise guarantee covers the use of ab64259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Mouse specific HRP/DAB (ABC) Detection IHC Kit images

  • Human colon carcinoma stained with anti p53 antibody using Anti Mouse HRP/DAB Detection Kit (ab64259).

References for Mouse specific HRP/DAB (ABC) Detection IHC Kit (ab64259)

This product has been referenced in:
  • Kim S  et al. Differentiation generates paracrine cell pairs that maintain basaloid mouse mammary tumors: proof of concept. PLoS One 6:e19310 (2011). IHC-P . Read more (PubMed: 21541292) »
  • Bhat A  et al. Differential growth factor adsorption to calvarial osteoblast-secreted extracellular matrices instructs osteoblastic behavior. PLoS One 6:e25990 (2011). IHC-P . Read more (PubMed: 21998741) »

See all 2 Publications for this product

Product Wall

Super easy to use, abtained nice image with it!

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This IHC kit is very easy to use, I get very clear images the first time I use it. It basically has everything you need for IHC staining except mouse primary antibody. Using counter staining will make the image look sharper, please be aware that the kit does not have hematoxylin for nuclear counter staining.
Username

Abcam user community

Verified customer

Submitted Nov 23 2016

I understand you used all of the 0.5 ml of pure 50x DAB chromogen.
As stated in the protocol booklet, the DAB chromogen must be diluted 50 times before being applied on the samples. See step 8 on page 6 of the protocol booklet: "Add 30 μl (...

Read More


I have contacted the lab who have developed this kit. They have confirmed that you are free to use your own anti-mouse HRP secondary in place of the Biotinylated Goat anti-mouse antibody and Streptavidin Peroxidase supplied in the kit, as scientif...

Read More

Thank you again for your feedback and comments.

We have now added hematoxylin to the catalog: ab128990.
http://www.abcam.com/Mayers-Hematoxylin-ab128990.html

As an apology to you for the inconvenience and an appreciation of y...

Read More

Thank you for your email.

I will pass on your suggestions to the according department in Abcam. Thank you so much for your feedback! We do appreciate this.

Please do not hesitate to contact us if you need any more advice or inform...

Read More

Thank you for contacting us.

Thewashing / rinsing buffer to be used with this kit isPBS/Tween 20.

We do carry this buffer in our catalog in 2 different sizes(125 ml and 1000 ml):

ab64028: http://www.abcam.com/20x-PBS-Buff...

Read More

DISCOUNT CODE: XXXXXXXXXXX
Expiration date: 21-09-2012

I am very pleased to hear you would like to accept our offer and test ab89566 in [IHC-P]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the...

Read More

Merci pour votre émail.

Je suis désolée que les résultats ne sont pas claires.

Je vais volontiers investiguer ces résultats et essayer de donner des conseils pour améliorer les images. Si un de nos produits serait défectueux, nous l...

Read More

Thank you for your reply.

If you change the country at the top right hand site of the Abcam website (http://www.abcam.com), you can see the country specific prices and also (in case of China) the distributor details.

Please be aware...

Read More

Thank you for your inquiry.

The reagents of this kit have to be used according to the size of the samples.

If you are using 100ul of the DAB mixed with DAB substrate per slide(this depends on the size of the sample on the slide, it ...

Read More

1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"