Overview

  • Product name
    Mouse TARC ELISA Kit (CCL17)
    See all TARC kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s mouse TARC (CCL17) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse TARC (CCL17)  in cell culture supernatants, serum and plasma (heparin, EDTA).

    A TARC (CCL17) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for TARC (CCL17)  is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse TARC (CCL17)  amount of sample captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    ABC Diluent Buffer 1 x 12ml
    Antibody Diluent Buffer 1 x 12ml
    Anti-mouse TARC Antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-mouse TARC antibody 1 x 130µl
    Lyophilized recombinant mouse TARC standard 2 x 10ng
    Plate Seal 1 x 4 units
    Sample Diluent Buffer 1 x 30ml
    TMB Color Developing Agent 1 x 10ml
    TMB Stop Solution 1 x 10ml
  • Research areas
  • Function
    Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4.
  • Tissue specificity
    Expressed at high levels in thymus and at low levels in the lung, colon and small intestine.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • A 152E5.3
    • ABCD 2
    • C C motif chemokine 17
    • C-C motif chemokine 17
    • CC chemokine TARC
    • CCL 17
    • CCL17
    • CCL17_HUMAN
    • Chemokine (C C motif) ligand 17
    • Chemokine ligand 17
    • MGC138271
    • MGC138273
    • SCYA17
    • Small inducible cytokine A17
    • Small inducible cytokine subfamily A (Cys Cys) member 17
    • Small inducible cytokine subfamily A member 17
    • Small-inducible cytokine A17
    • T cell directed CC chemokine
    • Thymus and activation regulated chemokine
    • Thymus and activation-regulated chemokine
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab171337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab171337

Protocols

References

ab171337 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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