Overview

  • Product nameMouse TECK ELISA Kit
    See all TECK kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    20.5 pg/ml - 2000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.42 84% - 107%
    Serum 94.26 82% - 105%
    Plasma 92.68 80% - 106%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s TECK Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TECK in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse TECK coated on a 96- well plate. Standards and samples are pipetted into the wells and TECK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TECK antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TECK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceThis gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified.
  • Alternative names
    • C C motif chemokine 25
    • CCL 25 MGC125074
    • CCL25
    • Chemokine (C C motif) ligand 25
    • Chemokine TECK
    • Ck beta 15
    • Ckb 15
    • Ckb15
    • MGC150327
    • SCY A25
    • SCYA 25
    • SCYA25
    • Small inducible cytokine A25
    • Small inducible cytokine A25 isoform 1 precursor
    • Small inducible cytokine A25 isoform 2 precursor
    • Small inducible cytokine subfamily A (Cys Cys) member 25
    • Small inducible cytokine subfamily A Cys Cys member 25
    • Small inducible cytokine subfamily A, member 25
    • TECKvar
    • Thymus expressed chemokine
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse TECK ELISA Kit images

  • Representative Standard Curve using ab100743

  • Representative Standard Curve using ab100743

Protocols

References for Mouse TECK ELISA Kit (ab100743)

ab100743 has not yet been referenced specifically in any publications.

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