Mouse Th1/Th2/Th17 (7 plex) Multiplex Immunoassay Kit (ab213395)

Overview

  • Product name
    Mouse Th1/Th2/Th17 (7 plex) Multiplex Immunoassay Kit
  • Detection method
    Flow cytometry-fluorescent
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Our multiplex immunoassays use the Firefly® particle technology to enable the quantitative measurement of many mouse proteins and peptides at the same time using as little as 12.5 μL of sample per well.


    The assay takes approximately 3.5 hours, followed by particle analysis using a validated flow cytometer model and then data analysis using our free Firefly Analysis Workbench software.


    Benefits of the assays:
    - Save time and conserve samples by measuring many analytes at the same time in each sample.
    - Easily build the right panel for your experiment with simple custom panel assembly, convenient pre-mixed pre-designed panels and the ability to add new analytes to pre-designed panels.
    - Run sensitive & accurate assays, built around antibody pairs that are validated with appropriate biological samples.
    - Use one platform for both multiplex miRNA and multiplex immunoassays with Firefly particle-based assays.


    Find out more about Firefly particle-based multiplex immunoassays.


    Important - Use of the Firefly Mouse Multiplex Immunoassay Focus panel requires the purchase of:
    - a) Multiplex Immunoassay Mouse Core Kit (ab209499); and
    - b) Multiplex Immunoassay Protein Standard Mix A (ab209500).


    Firefly® is a registered trade mark in the United States and is registered as a European Union Trade Mark.

  • Notes

    The following information provides the sensitivity, range, CVs, required Protein Standard Mix and links to the individual target kit, where more information regarding recommeneded dilutions can be found. 

    Target

    Sensitivity
    (pg/mL)
    Dynamic Range
    (pg/mL)

    Intra-assay
    CV

    Inter-assay
    CV

    Particle Code Protein
    Standard
    TNF alpha 3.14  4.6 - 10,000 6.2% 9.3%   1 Mix A
    IFN gamma 0.34  1.5 - 3,333  3.4% 5.6%  23  Mix A
    IL-2 1.21  4.6 - 10,000  2.7% 4.5%  Mix A
    IL-4 0.25  1.5 - 3,333  6.3% 3.1% Mix A
    IL-6 13.38  13.7 - 30,000  3.7% 2.5%  Mix A
    IL-10 3.7  13.7 - 30,000  6.7% 8.6%  13  Mix A
    IL-17A 0.21  1.5 - 3,333  3.8% 6.5%  17  Mix A

Properties

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References

ab213395 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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