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|Sample type||Average %||Range|
|Serum||111||110% - 113%|
|Cell culture extracts||104||90% - 119%|
|Cell culture media||107||106% - 108%|
|Citrate Plasma||112||109% - 113%|
TIMP1 in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TIMP1 protein in mouse serum, plasma, cell culture supernatant, cell and tissue extracts.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Samples diluted in Sample Diluent NS – 6.2 pg/mL
Samples diluted in 1X Cell Extraction Buffer PTR – 8 pg/mL
Tissue metalloproteinase inhibitor 1 (TIMP1or Metalloproteinase inhibitor 1) inhibits matrix metalloproteinases (MMP’s) which cause degradation of the extracellular matrix. TIMP1 inhibits MMP’s by forming a non-covalent bond complex at their catalytic zinc cofactor site and irreversibly inactivating them. TIMP1 also promotes cell proliferation and has some anti-apoptotic functions.
|Components||1 x 96 tests|
|10X Mouse TIMP1 Capture Antibody||1 x 600µl|
|10X Mouse TIMP1 Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|50X Cell Extraction Enhancer Solution (ab193971)||1 x 1ml|
|5X Cell Extraction Buffer PTR (ab193970)||1 x 10ml|
|ab221825 - Antibody Diluent 5BI||1 x 6ml|
|Mouse TIMP1 Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS (ab193972)||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab196265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of TIMP1 were measured in duplicate and interpolated from the TIMP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP1 concentration was determined to be 4,495 pg/mL in Mouse serum and 4,210 pg/mL in Mouse plasma.
Background subtracted data values (mean +/- SD, n = 2) are graphed.
L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. TIMP1 was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the TIMP1 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 4.1 ng/mL unstimulated, 18.5 ng/mL stimulated. There was no detectable signal in media.
ab196265 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"