Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesMouse
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab39315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Purity> 95 % by SDS-PAGE.
    This protein is purified from BHK cells transfected with full-length mouse TIMP-2, and is essentially free of contaminating MMPs or other known TIMPs.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, PBS, pH 7.4

General info

  • Alternative names
    • CSC 21K
    • CSC-21K
    • CSC21K
    • Metalloproteinase inhibitor 2
    • Metalloproteinase inhibitor 2 precursor
    • Metalloproteinase inhibitor 2 precursor
    • TIMP 2
    • TIMP 2
    • TIMP metallopeptidase inhibitor 2
    • TIMP-2
    • TIMP2
    • TIMP2_HUMAN
    • Tissue Inhibitor of Metalloproteinase 2
    • Tissue Inhibitor of Metalloproteinase 2
    • Tissue inhibitor of metalloproteinases 2
    see all
  • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Mouse TIMP2 full length protein (ab39315)

This product has been referenced in:
  • Lluri G  et al. Tissue inhibitor of metalloproteinase-2 (TIMP-2) regulates myogenesis and beta1 integrin expression in vitro. Exp Cell Res 314:11-24 (2008). Read more (PubMed: 17678891) »

See 1 Publication for this product

Product Wall

Application Western blot
PAGE: 12%, reducing-denaturing
1 ug total Timp2 protein loaded
Blocking with 10% milk overnight
Primary antibody: mouse anti-Timp2 abcam1828
Secondary antibody: biotinylated anti-mouse abcam ab97033
Detection: ABC system
Band at around 24 kDa
Username

Mrs. Henda Nabli

Verified customer

Submitted Oct 18 2013


Yes you can use ab39315 as a positive control for western blotting with the anti-Timp2 antibody ab1828.
There is a note on the online product datasheet for ab39315 with some further details, which states a western blot "Produces a band of app...

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Thank you for your enquiry and interest in our products. I can confirm that TIMP2 protein was isolated from mouse lung fibroblasts so the amino acid sequence corresponds to the murineTIMP2. According to the datasheet ab39315 has not been tested for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"