• Product nameMouse TNF alpha ELISA Kit
    See all TNF alpha kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 60 pg/ml
  • Range
    93.75 pg/ml - 6000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 92.46 84% - 104%
    Serum 94.18 82% - 103%
    Plasma 93.82 83% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s TNF alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF alpha in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • APC1 protein
    • Cachectin
    • DIF
    • soluble form
    • Tnf
    • TNF a
    • TNF macrophage derived
    • TNF monocyte derived
    • TNF superfamily member 2
    • TNF-a
    • TNF-alpha
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse TNF alpha ELISA Kit images

  • TNF? was measured in cell culture medium from Raw control cells, or cells stimulated with PMA (10 ng x mL-1; 24 h) and LPS (1 �g x mL-1; 6 h). Results are shown with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab100747

  • Representative Standard Curve using ab100747


References for Mouse TNF alpha ELISA Kit (ab100747)

This product has been referenced in:
  • Ribeiro EL  et al. Diethylcarbamazine attenuates the development of carrageenan-induced lung injury in mice. Mediators Inflamm 2014:105120 (2014). Read more (PubMed: 24550603) »
  • Razali FN  et al. Stimulatory effects of polysaccharide fraction from Solanum nigrum on RAW 264.7 murine macrophage cells. PLoS One 9:e108988 (2014). Plants . Read more (PubMed: 25299340) »

See all 4 Publications for this product

Product Wall

The ELISA assays are compatible with RIPA buffer. However, you should prepare your standards and positive controls in the same buffer.

The Bradford assay is compatible with RIPA buffer if the samples are diluted at least 10-fold with distill...

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Thank you for contacting us.

Although this kit has not been tested on lysates, it will likely work well using at least a 5 fold dilution of Assay Diluent B.

I hope this information is helpful to you. Please do not hesitate t...

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Thank you for contacting us.

Unfortunately, this kit’s cross reactivity with human has not been determined. If it helps, it looks like the homology of the extracellular domain between human and mouse is only around 70-77% which is not si...

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Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm that these three ELISA kits ab108870 ab100747 and ab100697 have been tested on mouse plasma and serum samples. (We would not recommend to test ...

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Thank you very much for your email and for sending your absorbance values.

I've reviewed this data with our lab, and we do agree that these standard absorbances are weaker than expected. Did yourun any samples as well?This would help narrow d...

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Thank you very much for your call today and for letting us know about the trouble with this new kit.

As we discussed, I'm sending a free of charge replacement kit on the order ***, which I expect to arrive Tuesday or Wednesday of next week. P...

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Abreview for TNF alpha Mouse ELISA Kit

Good Good 4/5 (Ease of Use)

Sample: Mouse Cell (Homogenised lung tissue)
Specification: Homogenised lung tissue
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: Virally infected lung tissue.
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Overall, a good kit. However, we struggled to detect TNFa at the highest concentration (6000pg/ml) due to too much signal when analysing at 450nm.

Tissue samples (5mg/ml) can be diluted between 1/5-1/100 and attain useful results.

Abcam user community

Verified customer

Submitted Aug 17 2012

Thank you for contacting us.

The following ELISA kits for mouse ICAM, VCAM, IL-6 and TNF-alpha are suitable for mouse samples:

ab100688 (http://www.abcam.com/ICAM1-Mouse-ELISA-Kit-1-x-96-Well-Plate-ab100688.html)
ab100713 (htt...

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Thank you for getting back to me and for sending us your data. I will forward this data and your feedback onto the lab.

Your credit note ID is ***.

I again apologize that thiskit did not perform as stated on the datasheet. As you ...

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Thank you for contacting me about this, and I am sorry to hear that the results were not as expected. I believe that the values given in my previous email were for normal plasma samples, but I will email the lab to check on that.

Could you ju...

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