Overview

  • Product name
    Mouse TrkB ELISA Kit
    See all TrkB kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Cell Lysate, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    93.8 pg/ml - 6000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s mouse TrkB in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse TrkB in cell culture supernatants, cell lysates and tissue homogenates.


    A TrkB specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for TrkB is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse TrkB amount of sample captured in plate.


    Get higher sensitivity in only 90 minutes with Mouse TrkB ELISA Kit (ab203368) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab119610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab119610

Protocols

References

ab119610 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

Unfortunately, this kit cannot be used with rat samples.

We have 2 TrkB antibodies (ab51190 and ab43079) that have been tested in indirect ELISA and rat samples, and might work together in a sandwich EL...

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Thank you for your enquiry.

I'll briefly describe the way our product discount testing program works.

1. Contact Abcam Scientific Support to discuss your novel use of the product
2. If we approve of your testing the product, yo...

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DISCOUNT CODE: ***
Expiration date: June 15th, 2012

I am very pleased to hear you would like to accept our offer and test ab119609 in mouse. This code will give you 1 free ELISA kit before the expiration date. To redeem this offer, pleas...

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Thank you for your patience.

The lab informed me thatRIPA buffer is compatible with the kit and can be usedwithtissue.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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