Overview

  • Product nameMouse Tweak ELISA Kit
    See all Tweak kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.3 pg/ml
  • Range
    0.27 pg/ml - 200 pg/ml
  • Recovery

    77 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.69 68% - 83%
    Serum 79.66 71% - 87%
    Plasma 77.72 70% - 89%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Tweak Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Tweak in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Tweak coated on a 96-well plate. Standards and samples are pipetted into the wells and Tweak present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Tweak antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Tweak bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab113320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse Tweak ELISA Kit images

  • Representative Standard Curve using ab113320.

Protocols

References for Mouse Tweak ELISA Kit (ab113320)

ab113320 has not yet been referenced specifically in any publications.

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This ELISA kit has not been tested against monomeric TWEAK/TNFSF12. It is only known to detect mouse TWEAK in its trimeric form. It is important to note that TWEAK is a homotrimer, mean...

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I have emailed the lab to replace the kit component item G (lot GR650572-2) which you had received empty. I will be in touch with more inform...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"