MOV10 may be an helicase with an important function in development and/or control of cell proliferation.
RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.
Detection of Human Mov10 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP Mouse NIH3T3 whole cell lysate loaded. ab176687 used for IP at 6 µg/mg lysate. For blotting immunoprecipitated Mov10, ab176687 was used at 1 µg/ml.