Overview

  • Product nameAnti-Mov10 antibody
    See all Mov10 primary antibodies
  • Description
    Rabbit polyclonal to Mov10
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: FCKENGGYTG CPFPAKLDLQ QGQNLLQGLS KLSPSTSGPH SHDYLPQERE, corresponding to C terminal amino acids 937-986 of Human Mov10

  • Positive control
    • Human liver.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab60132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 114 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20543829

Target

  • RelevanceMOV10 may be an helicase with an important function in development and/or control of cell proliferation. RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • DKFZp667O1423 antibody
    • FLJ32791 antibody
    • fSAP113 antibody
    • Functional spliceosome-associated protein 113 antibody
    • gb110 antibody
    • MGC2948 antibody
    • Moloney leukemia virus 10 protein antibody
    • MOV 10 antibody
    • Mov10 (Moloney leukemia virus 10, mouse) homolog antibody
    • Mov10 (Moloney leukemia virus 10,) homolog antibody
    • Mov10, Moloney leukemia virus 10, homolog (mouse) antibody
    • Mov10, Moloney leukemia virus 10, homolog antibody
    • Putative helicase MOV10 antibody
    see all

Anti-Mov10 antibody images

  • Anti-Mov10 antibody (ab60132) at 1.25 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution.

    Predicted band size : 114 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling Mov10 with ab60132 at 4-8µg/ml. Arrows indicate positively labelled hepatocytes. Magnification: 400X.

  • ICC/IF image of ab60132 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60132, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Mov10 antibody (ab60132)

This product has been referenced in:
  • Messaoudi-Aubert SE  et al. Role for the MOV10 RNA helicase in polycomb-mediated repression of the INK4a tumor suppressor. Nat Struct Mol Biol 17:862-8 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20543829) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Testis)
Loading amount 100 µg
Specification Testis
Gel Running Conditions Non-reduced Denaturing (10% PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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Verified customer

Submitted Feb 27 2012

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Unfortunately, this product has not been tested and is not guaranteed to work in mouse. In the future, please take advantage of our testing discount program for untested applications and species. Please visit ...

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