Overview

  • Product nameAnti-Mov10 antibody
    See all Mov10 primary antibodies
  • Description
    Rabbit polyclonal to Mov10
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 953 and 1003 of human Mov10 homolog (NP_066014.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibody was affinity purified using an epitope specific to MOV10 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • RelevanceMOV10 may be an helicase with an important function in development and/or control of cell proliferation. RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • DKFZp667O1423 antibody
    • FLJ32791 antibody
    • fSAP113 antibody
    • Functional spliceosome-associated protein 113 antibody
    • gb110 antibody
    • MGC2948 antibody
    • Moloney leukemia virus 10 protein antibody
    • MOV 10 antibody
    • Mov10 (Moloney leukemia virus 10, mouse) homolog antibody
    • Mov10 (Moloney leukemia virus 10,) homolog antibody
    • Mov10, Moloney leukemia virus 10, homolog (mouse) antibody
    • Mov10, Moloney leukemia virus 10, homolog antibody
    • Putative helicase MOV10 antibody
    see all

Anti-Mov10 antibody images

  • All lanes : Anti-Mov10 antibody (ab80613) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 114 kDa
    Observed band size : 114 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of HeLa whole cell lysate using ab80613 at 3µg/mg (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80613 at 1µg/ml.
    Detection: Chemiluminescence with an exposure time of 30 seconds.
  • ICC/IF image of ab80613 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80613, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Mov10 antibody (ab80613)

This product has been referenced in:

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% triton x100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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Verified customer

Submitted Sep 19 2012

Thank you for contacting us.

I am sorry to hear of the troubles which you have been experiencing using our products. Could please provide me with details of your western blotting protocol as well as tissue type and preparation steps and an...

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Thank you for your inquiry. Unfortunately, we do not know of a publication where this antibody was used in a co-IP experiment. I would like to reassure you that this antibody is tested and guaranteed for IP by our Abpromise. To co-IP a protei...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"