Overview

  • Product nameAnti-MOX1 antibody
    See all MOX1 primary antibodies
  • Description
    Rabbit polyclonal to MOX1
  • SpecificityPredicted to not crossreact with MOX2.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 19 amino acid peptide near the center of Human MOX1 (NP_004518).

  • Positive control
    • Human liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab105349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 28 kDa.

Target

  • FunctionMesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. Required for maintenance of the sclerotome polarity and formation of the cranio-cervical joints (PubMed:23290072, PubMed:24073994). Binds specifically to the promoter of target genes and regulates their expression. Activates expression of NKX3-2 in the sclerotome. Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner. Required for hematopoietic stem cell (HSCs) induction via its role in somitogenesis: specification of HSCs occurs via the deployment of a specific endothelial precursor population, which arises within a sub-compartment of the somite named endotome.
  • Involvement in diseaseKlippel-Feil syndrome 2, autosomal recessive
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus. Cytoplasm. Localizes predominantly in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeobox protein MOX-1 antibody
    • MEOX1 antibody
    • MEOX1_HUMAN antibody
    • Mesenchyme homeo box 1 antibody
    • Mesenchyme homeobox 1 antibody
    • MOX 1 antibody
    • MOX1 antibody
    see all

Anti-MOX1 antibody images

  • Immunofluorescence of MOX1 in rat liver tissue with ab105349 at 20 ug/mL.

  • Lane 1 : Anti-MOX1 antibody (ab105349) at 1 µg/ml
    Lane 2 : Anti-MOX1 antibody (ab105349) at 2 µg/ml

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 28 kDa

References for Anti-MOX1 antibody (ab105349)

ab105349 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E8.5 and E10.5 mouse embryo)
Specification E8.5 and E10.5 mouse embryo
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS/0.5%v/v TritonX100 (Sigma)
Blocking step 1%BSA+5%serum(depend on secondary antibody) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jan 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"