- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-MOX1 antibody
See all MOX1 primary antibodies -
DescriptionRabbit polyclonal to MOX1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Cow, Dog
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Immunogen
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Positive control
- Jurkat cell lysate. Human liver cells. Human stomach cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab23279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).Can be blocked with MOX1 peptide (ab199250). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionMesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. Required for maintenance of the sclerotome polarity and formation of the cranio-cervical joints (PubMed:23290072, PubMed:24073994). Binds specifically to the promoter of target genes and regulates their expression. Activates expression of NKX3-2 in the sclerotome. Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner. Required for hematopoietic stem cell (HSCs) induction via its role in somitogenesis: specification of HSCs occurs via the deployment of a specific endothelial precursor population, which arises within a sub-compartment of the somite named endotome.
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Involvement in diseaseKlippel-Feil syndrome 2, autosomal recessive
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Sequence similaritiesContains 1 homeobox DNA-binding domain.
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Cellular localizationNucleus. Cytoplasm. Localizes predominantly in the nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 4222 Human
- Omim: 600147 Human
- SwissProt: P50221 Human
- Unigene: 438 Human
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Alternative names
- Homeobox protein MOX-1 antibody
- MEOX1 antibody
- MEOX1_HUMAN antibody
see all
Images
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Western blot - MOX1 antibody (ab23279)Anti-MOX1 antibody (ab23279) at 0.5 µg/ml + Jurkat cell lysate
Secondary
HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000
Predicted band size: 28 kDa
Observed band size: 32 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Paraffin-embedded sections) - MOX1 antibody (ab23279)ab23279, at 4.0 - 8.0ug/ml staining human MOX1 in (human Liver) by Immunohistochemistry,Paraffin embedded tissue. Positive cells marked by arrows are hepatocytes. magnification 400 x. -
Immunohistochemistry (Paraffin-embedded sections) - MOX1 antibody (ab23279)ab23279, at 4.0 - 8.0ug/ml dilution, staining human MOX1 in human stomach by Immunohistochemistry, Paraffin embedded tissue. Cells with positive staining, marked by arrows, are Epithelial cells of Fundic Gland. Magnification 400 x.
References
ab23279 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"