- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-MOX1 antibody
See all MOX1 primary antibodies -
DescriptionRabbit polyclonal to MOX1
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide derived from a region between residues 42-96 of Human MOX1.
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Positive control
- Jurkat cell lysate. Human liver cells. Human stomach cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab23279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).Can be blocked with MOX1 peptide (ab199250). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionMesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. Required for maintenance of the sclerotome polarity and formation of the cranio-cervical joints (PubMed:23290072, PubMed:24073994). Binds specifically to the promoter of target genes and regulates their expression. Activates expression of NKX3-2 in the sclerotome. Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner. Required for hematopoietic stem cell (HSCs) induction via its role in somitogenesis: specification of HSCs occurs via the deployment of a specific endothelial precursor population, which arises within a sub-compartment of the somite named endotome.
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Involvement in diseaseKlippel-Feil syndrome 2, autosomal recessive
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Sequence similaritiesContains 1 homeobox DNA-binding domain.
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Cellular localizationNucleus. Cytoplasm. Localizes predominantly in the nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 4222 Human
- Omim: 600147 Human
- SwissProt: P50221 Human
- Unigene: 438 Human
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Alternative names
- Homeobox protein MOX-1 antibody
- MEOX1 antibody
- MEOX1_HUMAN antibody
see all
Anti-MOX1 antibody images
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Western blot - MOX1 antibody (ab23279)Anti-MOX1 antibody (ab23279) at 0.5 µg/ml + Jurkat cell lysate
Secondary
HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000
Predicted band size : 28 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Paraffin-embedded sections) - MOX1 antibody (ab23279)ab23279, at 4.0 - 8.0ug/ml staining human MOX1 in (human Liver) by Immunohistochemistry,Paraffin embedded tissue. Positive cells marked by arrows are hepatocytes. magnification 400 x. -
Immunohistochemistry (Paraffin-embedded sections) - MOX1 antibody (ab23279)ab23279, at 4.0 - 8.0ug/ml dilution, staining human MOX1 in human stomach by Immunohistochemistry, Paraffin embedded tissue. Cells with positive staining, marked by arrows, are Epithelial cells of Fundic Gland. Magnification 400 x.
References for Anti-MOX1 antibody (ab23279)
ab23279 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"