Overview

  • Product nameAnti-MOX1 antibody
    See all MOX1 primary antibodies
  • Description
    Rabbit polyclonal to MOX1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH, from the N terminal region of MOX1.

  • Positive control
    • 293 cell line lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 28 kDa.Can be blocked with MOX1 peptide (ab199249).
ICC/IF 1/10 - 1/50.

Target

  • FunctionMesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. Required for maintenance of the sclerotome polarity and formation of the cranio-cervical joints (PubMed:23290072, PubMed:24073994). Binds specifically to the promoter of target genes and regulates their expression. Activates expression of NKX3-2 in the sclerotome. Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner. Required for hematopoietic stem cell (HSCs) induction via its role in somitogenesis: specification of HSCs occurs via the deployment of a specific endothelial precursor population, which arises within a sub-compartment of the somite named endotome.
  • Involvement in diseaseKlippel-Feil syndrome 2, autosomal recessive
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus. Cytoplasm. Localizes predominantly in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeobox protein MOX-1 antibody
    • MEOX1 antibody
    • MEOX1_HUMAN antibody
    • Mesenchyme homeo box 1 antibody
    • Mesenchyme homeobox 1 antibody
    • MOX 1 antibody
    • MOX1 antibody
    see all

Anti-MOX1 antibody images

  • Immunofluorescence of HeLa cells labelling MOX1 with ab75895. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab75895 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃) and cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MOX1 immunoreactivity is localised to the nucleolus.

  • Anti-MOX1 antibody (ab75895) at 1/230 dilution + 293 cell line lysate at 35 µg

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References for Anti-MOX1 antibody (ab75895)

This product has been referenced in:
  • Thiaville MM  et al. Identification of PBX1 target genes in cancer cells by global mapping of PBX1 binding sites. PLoS One 7:e36054 (2012). Read more (PubMed: 22567123) »

See 1 Publication for this product

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