• Product nameAnti-MPG antibody
    See all MPG primary antibodies
  • Description
    Mouse monoclonal to MPG
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen




Our Abpromise guarantee covers the use of ab55461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • FunctionHydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
  • Sequence similaritiesBelongs to the DNA glycosylase MPG family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 alkyladenine DNA glycosylase antibody
    • 3-alkyladenine DNA glycosylase antibody
    • 3-methyladenine DNA glycosidase antibody
    • 3MG_HUMAN antibody
    • AAG antibody
    • ADPG antibody
    • Alkyladenine DNA glycosylase antibody
    • anpg antibody
    • APNG antibody
    • CRA36.1 antibody
    • DNA 3 methyladenine glycosylase antibody
    • DNA-3-methyladenine glycosylase antibody
    • MDG antibody
    • Mid1 antibody
    • Mpg antibody
    • N methylpurine DNA glycosirase antibody
    • N methylpurine DNA glycosylase antibody
    • N-methylpurine-DNA glycosylase antibody
    • PIG11 antibody
    • PIG16 antibody
    • Proliferation inducing protein 11 antibody
    • Proliferation inducing protein 16 antibody
    see all

Anti-MPG antibody images

  • Predicted band size : 33 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MPG knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab55461 observed at 35 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab55461 was shown to specifically react with MPG when MPG knockout samples were used. Wild-type and MPG knockout samples were subjected to SDS-PAGE. ab55461 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • MPG antibody (ab55461) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.

  • Predicted band size : 33 kDa
    MPG antibody (ab55461) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55461 staining MPG in human mammary tissue by Immunohistochemistry (paraffin embedded sections).
    Paraffin-embedded blocks were sectioned and mounted on frost-free slides. The 3-10 µm sections were deparaffinized in xylene and rehydrated through a series of graded alcohols. Slides were washed with 1× PBS and endogenous peroxidases were blocked with 1.5% hydrogen peroxide in 1× PBS for 20 minutes at 25°C. After three 5 minutes washes in 1× PBS, slides were incubated in blocking solution (1× PBS with 0.1% Triton X-100, 3% bovine serum albumin) with 5% normal donkey serum for 10 minutes at 25°C. Control (no primary antibody) and experimental slides were incubated overnight at 4°C, respectively, in blocking solution alone or blocking solution with ab55461 at 1/600 dilution. Biotin-conjugated secondary antibody 1/200 was added and slides were incubated at 25°C for 30 minutes and then washed three times with 1× PBS. The ABC Peroxidase Staining kit (1:100 dilution of each Reagent A

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa

    Exposure time : 10 seconds
  • Overlay histogram showing HeLa cells stained with ab55461 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55461, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemical analysis of Human gliomas, staining MPG with ab55461.

    Formalin fixed, paraffin-embedded tissue was blocked with 5% normal horse serum and incubated with primary antibody (1/500) overnight at 4°C. Staining was detected using DAB.

References for Anti-MPG antibody (ab55461)

This product has been referenced in:
  • Sousa MM  et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One 8:e55493 (2013). WB ; Human . Read more (PubMed: 23405159) »
  • Liu C  et al. Aberrant expression of N-methylpurine-DNA glycosylase influences patient survival in malignant gliomas. J Biomed Biotechnol 2012:760679 (2012). WB, IHC-P ; Human . Read more (PubMed: 22496614) »

See all 4 Publications for this product

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The final dilutions should be optimized for your particular samples and protocols, but the following would be my recommended starting dilutions for WB with an overnight incubation:

MGMT (ab39253) - 1:500...

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