Validated using a knockout cell line
Recombinant
RabMAb

Anti-MPG antibody [EPR10959(B)] (ab155092)

Overview

  • Product name
    Anti-MPG antibody [EPR10959(B)]
    See all MPG primary antibodies
  • Description
    Rabbit monoclonal [EPR10959(B)] to MPG
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human MPG (UniProt: P29372).

  • Positive control
    • HeLa, 293T and Jurkat cell lysates; Human testis tissue and ovarian carcinoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10959(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.

Use with paraformaldehyde fixed cells, permeabilized with 0.5% Triton X100/PBS.

WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function
    Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
  • Sequence similarities
    Belongs to the DNA glycosylase MPG family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 alkyladenine DNA glycosylase antibody
    • 3-alkyladenine DNA glycosylase antibody
    • 3-methyladenine DNA glycosidase antibody
    • 3MG_HUMAN antibody
    • AAG antibody
    • ADPG antibody
    • Alkyladenine DNA glycosylase antibody
    • anpg antibody
    • APNG antibody
    • CRA36.1 antibody
    • DNA 3 methyladenine glycosylase antibody
    • DNA-3-methyladenine glycosylase antibody
    • MDG antibody
    • Mid1 antibody
    • Mpg antibody
    • N methylpurine DNA glycosirase antibody
    • N methylpurine DNA glycosylase antibody
    • N-methylpurine-DNA glycosylase antibody
    • PIG11 antibody
    • PIG16 antibody
    • Proliferation inducing protein 11 antibody
    • Proliferation inducing protein 16 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MPG knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab155092 observed at 35 kDa. Red - loading control, ab8245.

    ab155092 was shown to specifically react with MPG when MPG knockout samples were used. Wild-type and MPG knockout samples were subjected to SDS-PAGE.  Ab155092 and ab8245 (loading control to GAPDH) were diluted at 1/10,000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155092 showing +ve staining.

  • All lanes : Anti-MPG antibody [EPR10959(B)] (ab155092) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 33 kDa

  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MPG with unpurified ab155092 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MPG with ab155092 at 1/50 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human tesis tissue labeling MPG with ab155092 at 1/50 dilution.
  • Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155092 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155092 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155092 showing +ve staining.

  • ab155092 staining MPG in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab155092 (1/500) staining MPG in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5%Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

References

This product has been referenced in:
  • Venkatachalam G  et al. Replication stress-induced endogenous DNA damage drives cellular senescence induced by a sub-lethal oxidative stress. Nucleic Acids Res 45:10564-10582 (2017). Read more (PubMed: 28985345) »
  • Khoronenkova SV & Dianov GL ATM prevents DSB formation by coordinating SSB repair and cell cycle progression. Proc Natl Acad Sci U S A 112:3997-4002 (2015). WB ; Human . Read more (PubMed: 25775545) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (Human primary fibroblasts)
Specification
Human primary fibroblasts
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 26 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-16% gradient Tris-Gly gel)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Blocking step
LI-COR­ Odyssey­ Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-16% gradient Tris-Gly gel)
Sample
Human Cell lysate - whole cell (TIG-1 primary fibroblasts)
Specification
TIG-1 primary fibroblasts
Blocking step
(agent) for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Feb 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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