Western blot - Anti-MPG antibody [EPR10959(B)] (ab155092)
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: MPG knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg) Lane 4: HEK293 cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab155092 observed at 35 kDa. Red - loading control, ab8245.
ab155092 was shown to specifically react with MPG when MPG knockout samples were used. Wild-type and MPG knockout samples were subjected to SDS-PAGE. Ab155092 and ab8245 (loading control to GAPDH) were diluted at 1/10,000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MPG with unpurified ab155092 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155092 showing +ve staining.
Immunocytochemistry/ Immunofluorescence - Anti-MPG antibody [EPR10959(B)] (ab155092)This image is courtesy of an anonymous Abreview
ab155092 staining MPG in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MPG antibody [EPR10959(B)] (ab155092)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab155092 (1/500) staining MPG in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5%Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
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