The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 78 kDa).
Phosphorylated in M (mitotic) phase.
Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus membrane. Localizes to the distal and proximal end of centriole pairs in duplicated centrosomes. In ciliated cells, localizes to the distal and proximal end of daughter centriole and proximal of the mother centriole but not in the distal end of the mother centriole.