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Abcam’s anti-MPO (p-ANCA) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgG class autoantibodies against myeloperoxidase (MPO) in Human serum and plasma.
A 96-well plate has been precoated with Human neutrophil myeloperoxidase antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized MPO-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of MPO IgG sample captured in plate.
Get results in 90 minutes with Human Myeloperoxidase ELISA Kit (ab195212) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|10X Wash Solution||1 x 50ml|
|Cover Foil||1 unit|
|IgG Sample Diluent||1 x 100ml|
|MPO Anti-IgG HRP Conjugate||1 x 15ml|
|MPO Coated Microplate (12 x 8 wells)||1 unit|
|MPO Negative Control||1 x 1.2ml|
|MPO Positive Control||1 x 1.2ml|
|MPO Standard 0 - 0 U/mL||1 x 1.2ml|
|MPO Standard 1 - 10 U/mL||1 x 1.2ml|
|MPO Standard 2 - 20 U/mL||1 x 1.2ml|
|MPO Standard 3 - 40 U/mL||1 x 1.2ml|
|MPO Standard 4 - 160 U/mL||1 x 1.2ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab178630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
Representative standard curve using ab178360
ab178630 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"