Anti-MPP2/DLG2 antibody (ab231634)
Key features and details
- Rabbit polyclonal to MPP2/DLG2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MPP2/DLG2 antibody
See all MPP2/DLG2 primary antibodies -
Description
Rabbit polyclonal to MPP2/DLG2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Mouse MPP2/DLG2 aa 1-250. (Expressed in E.coli).
Database link: Q9WV34 -
Positive control
- WB: Mouse cerebrum and kidney lysates; Recombinant mouse MPP2/DLG2 protein. IHC-P: Mouse esophagus tissue. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231634 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 62 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 - 20 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 62 kDa. |
Target
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Relevance
MPP2/DLG2 (Palmitoylated membrane protein 2) is a member of a family of membrane associated proteins termed MAGUKs (membrane associated guanylate kinase homologs). These proteins interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP2/DLG2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. -
Cellular localization
Cell Membrane; integral membrane protein -
Database links
- Entrez Gene: 4355 Human
- Entrez Gene: 50997 Mouse
- Entrez Gene: 85275 Rat
- Omim: 600723 Human
- SwissProt: Q14168 Human
- SwissProt: Q9WV34 Mouse
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Alternative names
- D11BWG0652E antibody
- Discs Large Homolog 2 antibody
- DLG2 antibody
see all
Images
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A4549 (Human lung carcinoma cell line) cells stained for MPP2/DLG2 using ab231634 at 20 µg/ml in ICC/IF.
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Formalin-fixed, paraffin-embedded mouse esophagus tissue stained for MPP2/DLG2 using ab231634 at 10 µg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded mouse esophagus tissue stained for MPP2/DLG2 using ab231634 at 10 µg/ml in immunohistochemical analysis.
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Anti-MPP2/DLG2 antibody (ab231634) at 2 µg/ml + Mouse kidney lysate
Predicted band size: 62 kDa -
Anti-MPP2/DLG2 antibody (ab231634) at 2 µg/ml + Mouse cerebrum lysate
Predicted band size: 62 kDa -
Anti-MPP2/DLG2 antibody (ab231634) at 2 µg/ml + Recombinant mouse MPP2/DLG2 protein
Predicted band size: 62 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab231634 has been referenced in 3 publications.
- Di Z et al. Single-cell and WGCNA uncover a prognostic model and potential oncogenes in colorectal cancer. Biol Proced Online 24:13 (2022). PubMed: 36117173
- Stepan J et al. Hippo-released WWC1 facilitates AMPA receptor regulatory complexes for hippocampal learning. Cell Rep 41:111766 (2022). PubMed: 36476872
- Li S et al. Circ0106714 inhibits tumorigenesis of colorectal cancer by sponging miR-942-5p and releasing DLG2 via Hippo-YAP signaling. Mol Carcinog 59:1323-1342 (2020). PubMed: 33128289