Overview

  • Product name
  • Description
    Goat polyclonal to MPP5
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    NKASPPFPLISNAQ

    , corresponding to N terminal amino acids 174-187 of Human MPP5.

  • Positive control
    • Human Brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab20511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ELISA 1/32000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 77 kDa).

Target

  • Function
    May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton.
  • Tissue specificity
    Expressed in the retina (at protein level).
  • Sequence similarities
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 2 L27 domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SH3 domain.
  • Domain
    The L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
    The PDZ domain binds to the C-terminus of SC6A1.
  • Cellular localization
    Cell membrane. Endomembrane system. Cell junction > tight junction. Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ12615 antibody
    • MAGUK p55 subfamily member 5 antibody
    • Membrane protein palmitoylated 5 antibody
    • MPP 5 antibody
    • MPP5 antibody
    • MPP5_HUMAN antibody
    • PALS 1 antibody
    • PALS1 antibody
    • Protein associated with Lin-7 antibody
    • Stardust (Drosophila) antibody
    • Stardust antibody
    see all

Images

  • Anti-MPP5 antibody (ab20511) at 1 µg/ml + Human brain lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 77 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ab20511 at 5ug/ml staining MPP5 in human skeletal tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

References

ab20511 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic dorsal forebrain)
Permeabilization
Yes - Triton 0.5%
Specification
Embryonic dorsal forebrain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Aug 10 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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