Overview

  • Product nameAnti-MPP5 antibody
    See all MPP5 primary antibodies
  • Description
    Rabbit polyclonal to MPP5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (AVDCPGDLGTRMMPIRRSAQLERIRQQQEDMRRRREEEGKKQELDLNSS M) of Human MPP5 (NP_071919)

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton.
  • Tissue specificityExpressed in the retina (at protein level).
  • Sequence similaritiesBelongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 2 L27 domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SH3 domain.
  • DomainThe L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
    The PDZ domain binds to the C-terminus of SC6A1.
  • Cellular localizationCell membrane. Endomembrane system. Cell junction > tight junction. Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ12615 antibody
    • MAGUK p55 subfamily member 5 antibody
    • Membrane protein palmitoylated 5 antibody
    • MPP 5 antibody
    • MPP5 antibody
    • MPP5_HUMAN antibody
    • PALS 1 antibody
    • PALS1 antibody
    • Protein associated with Lin-7 antibody
    • Stardust (Drosophila) antibody
    • Stardust antibody
    see all

Anti-MPP5 antibody images

  • Anti-MPP5 antibody (ab83891) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 40,77 kDa (why is the actual band size different from the predicted?)

References for Anti-MPP5 antibody (ab83891)

ab83891 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply and all the additional information. This makes the problems you have been experiencing much clearer to understand. It is clear that you have spent a lot of time in trying to optimise these antibodies and I am sorry that they ar...

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