Overview

  • Product name
  • Description
    Rabbit polyclonal to MPP8
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 305-417 of Human MPP8.

  • Positive control
    • Human testis tissue; U 2 OS cells; Human tonsil, RT 4 and U 251 MG cell line lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/250 - 1/500.

Target

  • Sequence similarities
    Contains 4 ANK repeats.
    Contains 1 chromo domain.
  • Post-translational
    modifications
    Phosphorylated in M (mitotic) phase.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSMPP8 antibody
    • M-phase phosphoprotein 8 antibody
    • M-phase phosphoprotein, mpp antibody
    • M-phase phosphoprotein, mpp8 antibody
    • MPHOSPH8 antibody
    • MPP8_HUMAN antibody
    • RP11-523H24.1 antibody
    • Twa3 antibody
    • Two hybrid-associated protein 3 with RanBPM antibody
    see all

Anti-MPP8 antibody images

  • ab122575, at 1/500 dilution, staining MPP8 in paraffin-embedded Human testis tissue by Immunohistochemistry.
  • ab122575, at 4 µg/ml, staining MPP8 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
  • All lanes : Anti-MPP8 antibody (ab122575) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References for Anti-MPP8 antibody (ab122575)

ab122575 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122575.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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