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Rabbit polyclonal to MPP8
Predicted to work with:
Horse, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide within Human MPP8 aa 400-500 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q99549
This antibody gave a positive signal in MCF7 Nuclear and HEK293 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 97 kDa).
Contains 4 ANK repeats.
Contains 1 chromo domain.
Phosphorylated in M (mitotic) phase.
Information by UniProt
M-phase phosphoprotein 8 antibody
M-phase phosphoprotein, mpp antibody
Western blot - Anti-MPP8 antibody (ab124499)
All lanes :
Anti-MPP8 antibody (ab124499) at 1 µg/ml
Lane 1 : MCF7 nuclear extract lysate ( ab14860) Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) ( ab97051) at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 97 kDa Observed band size: 125 kDa ( why is the actual band size different from the predicted?) Additional bands at: 80 kDa (possible non-specific binding) Exposure time: 2 minutes
The predicted molecular weight of MPP8 is 97 kDa (SwissProt), however we expect to observe a banding pattern around 125 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab124499 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution
has not yet been referenced specifically in any publications.
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